VIU
  • Links 
  • Emergency Info
  • Library
  • Campuses
    • Nanaimo
    • Parksville-Qualicum
    • Cowichan
    • Powell River
  • Programs and Courses
  • Directories
    • Employee Directory
    • Instructional Departments
    • Service Departments
  • Contact Us
    • Admin Login
    View Item 
    •   VIURRSpace Home
    • VIUSpace
    • Student Work
    • Biology
    • Biology 491 Senior Projects
    • View Item
    •   VIURRSpace Home
    • VIUSpace
    • Student Work
    • Biology
    • Biology 491 Senior Projects
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Optimization of media and transfection with SV40 large T antigen and c-myc for the establishment of an immortal sea urchin cell line

    Thumbnail
    View/Open
    Undergraduate Research Paper (588.6Kb)
    Date
    8/4/2010
    Author
    Stechishin, Owen
    Metadata
    Show full item record
    Abstract
    Immortal cell lines are cell cultures capable of indefinite growth without reaching senescence. Although many vertebrate and some terrestrial invertebrate cell lines have been produced, the establishment of an immortal marine invertebrate cell line remains an elusive goal. In general, marine invertebrate cell culture is greatly hindered by the lack of proliferation and long term viability of cultured cells. As such, much work in this field has focused on identifying media supplements and source cells amenable to long-term culture as well as optimizing immortalization methods. We surveyed seven media supplements (fetal bovine serum, newborn calf serum (NCS), horse serum, chicken serum, two commercial lipid mixtures and coelomic fluid) for their abilities to promote proliferation and long-term viability in culture. We also experimented with embryonic source tissues and developed an oncogene-mediated immortalization technique in hopes of producing transgenic marine invertebrate cells with greater proliferative capacity and decreased fastidiousness. We report that 1% supplementation of Leibowitz’s L-15 media with NCS or chicken serum afforded enhanced viability of coelomocytes as indicated by esterase activity and long term cell-to-substratum attachment compared to unsupplemented L-15 (statistical significance achieved for NCS; p<0.05, Duncan’s multiple range test). We also observed that embryonic tissue was capable of in vitro proliferation, but had significant contamination with protists, leading to rapid demise of cultures within 2 weeks. We observed the formation of colonies in coelomocyte cultures transfected with c-myc and SV40 large T antigen and report stable integration of the c-myc gene sequence in one cell culture five weeks after transfection; however, dramatically increased proliferation was not apparent over a 7 week period.
    URI
    http://hdl.handle.net/10613/63
    Collections
    • Biology 491 Senior Projects

    Browse

    All of VIURRSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Admin LoginRegister
    Social Media
    • Facebook
    • Instagram
    • LinkedIn
    • RSS
    • Twitter
    • YouTube
    Campus Services
    • Bookstore
    • Food Services
    • Discovery Room Restaurant
    • Employee Directory
    • Find VIU Experts
    • Event Services
    • Campus Caterers
    • Parking and Security
    • Service Departments
    Public Engagement
    • Advancement and Alumni
    • Communications and Public Engagement
    • Government Relations
    • University Relations
    Faculties
    • Academic & Career Preparation
    • Arts & Humanities
    • Education
    • Health & Human Services
    • International Programs
    • Science & Technology
    • Social Sciences
    • Management
    • Trades & Applied Technology
    Organizational Structure
    • Governance, Administration, & Planning
    • Instructional Departments
    • Provost, and VP Academic
    • Policies & Procedures
    • President's Office
    • University Relations
    • VP Admin and Finance
    • VIU Organization Chart
    Publications
    • Regional Strategy Plan
    • VIU Impact Report
    • VIU Report to the Community
    • Adding Value to Your Community
    Contact Info

    Vancouver Island University
    Nanaimo Campus
    900 Fifth Street
    Nanaimo, BC
    Canada V9R 5S5
    Toll-free 1.888.920.2221
    Switchboard 250.753.3245
    Email info@viu.ca

    Contact Us
    Send Feedback 
    Copyright ©
    Vancouver Island University

    About VIU
    • Administration
    • Advancement
    • Alumni Association
    • Cowichan Campus
    • Parksville-Qualicum Centre
    • Powell River Campus
    • Governance
    • Integrated Planning
    • Employment
    • Mission
    • History
    ACADEMICS
    • Admissions
    • Registration
    • Programs and Courses
    • Dual Credit
    • Financial Aid and Awards
    • Graduate Programs
    • International Education
    • Library
    • Online Education
    ATHLETICS
    • VIU Mariners
    • Mariners Teams
    • Campus Recreation
    • Summer Camps
    CAMPUS LIFE
    • Campus Store
    • Counseling
    • Disability Services
    • Food Services
    • Health and Wellness
    • Housing
    • Join One
    • Services for Aboriginal Students
    • Sustainability
    • Parking
    • Events
    Extension and Outreach
    • Contract Training
    • Professional Development and Training Courses
    • Summer Session
    • ElderCollege
    • GrandKids University
    • Youth Summer Camps
     
    DSpace Express is a service operated by 
    Atmire NV